Substrate Specificity of Soybean Lipoxidase
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چکیده
منابع مشابه
Substrate specificity of soybean lipoxidase.
Purified soybean lipoxidase was used to test the substrate specificity of all cis,ck-methylene-interrupted isomers of linoleic acid. The natural 9, U-isomer was found to be the best substrate, and the 13,16-isomer 50% as effective. The presence of calcium ions broadened the pattern of specificity. The rates of reaction of a series of polyunsaturated acids in which chain length, number of double...
متن کاملOn the specificity of the oxygenation of unsaturated fatty acids catalyzed by soybean lipoxidase.
The positional and stereochemical specificities of the soybean lipoxidase-catalyzed oxygenation of unsaturated fatty acids have been investigated. It was found that the oxygen function was introduced at position ~6 in all of the fatty acids which reacted. In one case a small amount of the substrate was oxygenated in position ~10. The structural requirement for substrates is the presence of a ci...
متن کاملStudies on the Substrate Specificity of Soybean Lipoxygenase - 1 Using an Entire
Substrate recognition mechanism o f soybean lipoxygenase-1 (L -l) was studied by using an entire series o f (co3Z,co6Z,co9Z)-C12~ C 22-trienoic acids and -trienols. The m axim um activity o f L-l was obtained with C20-trienoic acid o f all the substrates used. A m ong trienols, the m axi mum activity was obtained with C ,5-trienol. Analysis o f the products obtained by the reaction o f these s...
متن کاملInduction period in the lipoxidase-catalyzed oxidation of linoleic acid and its abolition by substrate peroxide.
The non-enzymatic oxidation of linoleate occurs with a definite induction period. Bolland has explained the kinetics of the oxidation in terms of a chain reaction involving free radicals (1). The discovery by Privett et al. (2) that the peroxide formed by the action of lipoxidase is optically active clearly rules out the possibility of a non-enzymatic chain sequence, but it is not impossible th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91821-1